AAVrg Ef1a-DIO-hChR2(H134R)-EYFP (30 µl)

AAVrg-EF1α-DIO-hChR2(H134R)-EYFP is a pre-packaged rAAV in retrograde serotype (with capsid from AAV serotype 2-retrograde and 2xITR from AAV serotype 2) which will Cre-dependently over-express hChR2(H134R)-EYFP under the EF1a as illustrated as the cartoon below. Ready to use format.

In the DIO scenario, the transgene of interest is inserted in reverse orientation relative to the 5' promoter and is flanked by oppositely oriented loxP and lox2272 sites. In the absence of Cre expression, the transgene will not be produced. In the presence of Cre expression, the transgene will be "Flip-exchanged" or FLEXed, leading to the expression of the transgene. This is due to a permanent Cre-mediated recombination/inversion of the flanked transgene. This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev (reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV).